DANA D. WISE

Present:
6805 Woodhollow Dr.
Austin TX 78731
d2wise@mail.utexas.edu

Permanent:
#354 930 Elizabeth Ct.
Cuyahoga Falls, OH 44221

EDUCATION
Ph.D. in progress (expected spring 2005), Analytical Chemistry. University of Texas at Austin. GPA: 4.00. Coursework: Analytical Chemistry, Optical Methods and Laser Spectroscopy, Neuroscience I and II, Neuropharmacology, Behavioral Neuroendocrinology. Advisor: Dr. Jason Shear.

Honors B.S. Chemistry, summa cum laude, 2000. University of Toledo, Ohio. GPA: 3.94. Honors Thesis: “Analysis of a Cholinergic System by Capillary Electrophoresis with Electrochemical Detection Using an Internal Standard Method.” Advisor: Dr. Jon R. Kirchhoff.

HONORS AND AWARDS
National Science Foundation Graduate Research Fellowship Honorable Mention, 2001.
The Outstanding Senior in Chemistry Award, University of Toledo, 2000.
Arthur H. Black Award in Analytical Chemistry, University of Toledo, 2000.
Analytical Chemistry Award (first in class), University of Toledo, 1999.
Biochemistry Award (first in class), University of Toledo, 1999.
Repeat President’s and Dean’s List Member, University of Toledo, 1996-2000.

FELLOWSHIPS AND GRANTS
Dorothy Banks Research Fellowship, University of Texas, 2004-2005 ($12,000 stipend).
Scholar Award, Philanthropy and Education Organization International, 2003-2004 ($9,000 stipend).
Welch Summer Research Fellowship, University of Texas, 2002 ($2,500 stipend).
Award of Excellence, Honor Society of Phi Kappa Phi, 2000 ($1,000 stipend).
Undergraduate Summer Research Fellowship, University of Toledo Honors Dept., 1998 ($2000 stipend, $250 supplies).
Sullivan Fellowship Award, University of Toledo Honors Dept., 1999 ($2000 stipend).
Sullivan Small Grants and Supplies Award, University of Toledo Honors Dept., 1999. ($250 for supplies).

RESEARCH EXPERIENCE

University of Texas, 2001-present.
Advisor: Dr. Jason Shear, Department of Chemistry.
Developed and exhaustively characterized a method for the extraction of vitamin B3 derivatives from cultured neural cells. Modified and systemized a capillary electrophoresis/laser-induced fluorescence instrument to separate, detect, and quantify these analytes. Tracked B3-derivative levels in both a control neural cell line and a “biological clock” neural cell line over 24 - 48 hour periods, to investigate putative rhythmicity and time-dependent variations.
Current research focuses on tracking the differentiation of cultured raphe nucleus neurons. Adapted the above method to also quantify serotonin and related chemicals over days of differentiation, while monitoring poly-ADP-ribose formation.

Cold Spring Harbor Laboratory, NY, June 5-25, 2002.
Professors: Michael Hausser, Nelson Spruston, Thomas Otis, Laurence Trussell
Completed “Physiological Approaches to Ion Channel Biology,” an intensive laboratory/lecture full-immersion short course in electrical recording from neural cells

University of Toledo, 1999-2000.
Advisor: Dr. Jon R. Kirchhoff, Department of Chemistry.
Prepared and characterized enzyme-modified microelectrodes in a capillary electrophoresis system. Developed technique for separation and detection of neurochemicals, using novel internal standard method. Prepared samples from rat brain tissue for analysis in the above system.

University of Toledo, 1996-1999.
Advisor: Dr. William Messer, Department of Medicinal and Biological Chemistry.
Completed a seven-step synthesis and isolation of a racemically pure pharmaceutical compound for the treatment of Alzheimer’s Disease.

TEACHING EXPERIENCE
Advanced Analytical Chemistry Laboratory, Spring 2001. Lectured, graded lab reports, held office hours, and supervised lab for small group of mid- to upper-class students, covering basic principles of analytical chemistry.

Analytical Chemistry Laboratory, Fall 2000. As above, but also mentored students through a writing-intensive independent project. Course emphasized analytical instrumentation, such as FTIR, NMR, MS, HPLC, GC, CE, AA/AE, UV-Vis and fluorescence spectroscopy.

RESEARCH PUBLICATIONS
“Circadian Tracking of Nicotinamide Cofactor Levels in an Immortalized Suprachiasmatic Nucleus Cell Line.” D.D. Wise and J.B. Shear. Neuroscience. 128 (2004) 263-268.

“Tracking Variations in Nicotinamide Cofactors Extracted from Cultured Cells Using Capillary Electrophoresis with Multiphoton Excitation of Fluorescence,” D. D. Wise and J. B. Shear. Analytical Biochemistry. 326 (2004) 225-233.

“An Internal Standard Method for the Measurement of Choline and Acetylcholine by Capillary Electrophoresis with Electrochemical Detection.” D.D. Wise, T.V. Sorokina, P.-A. Brault, J.R. Kirchhoff, R.A. Hudson, W.S. Messer. Journal of Chromatography B. 775 (2002) 49-56.

POSTERS AND PRESENTATIONS
“Circadian Tracking of Nicotinamide Cofactor Levels in an Immortalized Suprachiasmatic Nucleus Cell Line.” (poster) D.D. Wise, J.B. Shear. Society for Neuroscience 34th Annual Meeting. San Diego, CA. 10/2004.

“Tracking Circadian Variations in Nicotinamide Cofactor Levels in Cultured Cells.” D.D. Wise, J.B. Shear. Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy (PittCon). Chicago, IL. 3/2004.

“Capillary Electrophoresis with Laser-Induced Fluorescence for the Analysis of Nicotinamide Cofactors; Application to Suprachiasmatic Nucleus Cells.” (poster) D.D. Wise, J.B. Shear. Society for Neuroscience 32nd Annual Meeting. Orlando, FL. 11/2002.

“Capillary Electrophoresis with Electrochemical Detection for the Analysis of Choline and Acetylcholine.” D.D. Wise, J.R. Kirchhoff, W.S. Messer, R.A. Hudson. National Conference for Undergraduate Research, Missoula, MT. 4/2000.

“Determination of Choline and Acetylcholine by Capillary Electrophoresis with Electrochemical Detection Using an Internal Standard Method.” D.D. Wise, J.R. Kirchhoff, W.S. Messer, R.A. Hudson. PittCon. New Orleans, LA. 3/2000.

“New Methods in Evaluating Cholinergic Toxicity.” D.D. Wise, J.R. Kirchhoff. Honors Dept. “Brown Bag” lunch seminar, 3/2000.

“Synthesis of Selective Muscarinic Agonists for the Treatment of Alzheimer’s Disease.” D.D. Wise, Y. Cao, W.S. Messer. National Conference for Undergraduate Research, Rochester, NY. 3/1999.

“Keeping Minds Right with Right-Handed Molecules.” D.D. Wise, W.S. Messer. Honors Dept. “Brown Bag” lunch seminar, 10/1998.

RESEARCH CONTRIBUTIONS
“Analyte Recognition, Concentration, and Electrophoretic Separation Using Chemically Modified Microspheres.” M. Plenert (poster), D.D. Wise, M.L. Gostkowski, J.B. Shear. 24th International Symposium on Capillary Chromatography and Electrophoresis. Las Vegas, NV. 5/2001.

“Selective Detection at Biocatalytic Membrane Electrodes.” J.R. Kirchhoff (presenting), T. Inoue, D.D. Wise. Cincinnati Meeting of the American Chemical Society, Covington, KY. 5/2000.

“Activity of Selective Muscarinic Agonists at Human M1 Receptors Expressed at Varying Levels in A9L Cells.” Y. Cao (presenting), D.D. Wise, H. Zhang, X.-P. Huang, and W.S. Messer. The 8th Symposium on Subtypes of Muscarinic Receptors. Danvers, MA. 8/1998.
--Abstract of above published in Subtypes of Muscarinic Receptors VIII, Life Sciences, 64 (1999) p. 571.

ACTIVITIES AND AFFILIATIONS
Union of Concerned Scientists, 5/2004-present.
P.E.O International, 2003.
(invited speaker to local chapter CR: “Analysis of Metabolic Molecules in ‘Biological Clock’ Cells,” 9/2003).
(invited speaker to local chapter R, as above, 4/2004)
Society for Neuroscience, 2002-present.
University of Texas Chemistry Department High School Outreach Program, Tour Guide, 4/2002.
Honor Society of Phi Kappa Phi, 2000-present

CERTIFICATIONS
University of Texas
Intellectual Property (Copyright) Tutorial, 2004.
Laboratory Safety, 2000.
Laser Safety, 2001.
University of Toledo Animal Care Program
Humane Care and Use of Animals, 2000.
Biomethodology of Common Laboratory Animals, 2000.

REFERENCES
Professor Jason B. Shear, University of Texas at Austin, jshear@mail.utexas.edu
Professor Jon R. Kirchhoff, University of Toledo, Jon.Kirchhoff@utoledo.edu